Wednesday, December 26, 2018

Reflected Light Microscope

Reflected Light Microscope


Half Mirror

Partially reflecting plane glass mirror that deflects light traveling from the horizontal illuminator by 90 degrees into the vertical optical train of imaging components in the microscope. 

principle

Objective Lens 

•A matching well-corrected condenser properly aligned 
•An image-forming objective projecting the image-carrying rays toward the eyepiece 

Dark Field vs.Bright Field

Bright field:
•“normal”wide-field illumination method 
•bright background 
•low contrast

bright fild

Dark field: 

•an opaque disc is placed underneath the condenser lens 
•scattered light 
•dark background 
•high contrast (structural details)

dark fild



Phase Contrast Microscope


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