Thursday, December 27, 2018

Limitation in Optical Microscopy

Limitation in Optical Microscopy

Resolution limited by wavelength of light (diffraction)
limitation

Numerical Aperture

numeric

Stimulated Emission Depletion (STED) Microscopy

Prof. Stefan W. Hell (Max Planck Institute for Biophysical Chemistry)

sted
•The excitation spot is ~200 nm by focusing with a lens •A STED beam (doughnut-shaped and centered over the excitation spot) is used to quench the fluorescent markers before they fluoresce •Very smaller effective fluorescence spot (~60 nm)


Resolution Enhancement using STED

resulation

 Scanning Probe Microscopy




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