Thursday, December 27, 2018

Laser Scanning ConfocalMicroscope

Laser Scanning ConfocalMicroscope

Scanninga diffraction-limited point of excitation light across the sample
sample
The out of focus light rays are eliminated from the image by the use of a confocal“pinhole”
principle
Important technique for live cell and tissue imaging, the studies of biochemical dynamics!

Advantages:
•Optical sectioning ability 
•3D reconstruction 
•Excellent resolution (0.1-0.2 μm) •Specific wavelengths of light used 
•Very high sensitivity 
optical

Drawbacks
•Expensive 
•Complex to operate 
•Chemical labeling 
•High intensity laser light

Advantages of ConfocalMicroscope

advantages

MultiphotonMicroscope

Advantages:

•Fluorescence only occurs at the focal point
 •Able to image deeper into tissue sample 
advantages
Drawbacks: 
•Even more expensive (pulsed laser) 
•Localized heating (photobleaching)
drowbacks

Limitation in Optical Microscopy




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